Fermentor|bioreactor（500Liters ,Mechanical sealing system at the bootom of the fermenter;Double mechanical Seal） Model:KNIKBIO -500SBA
Total volume:500 liters
Working volume: 150L -400 liters
Material: 316L stainless steel with material contact to the other 304 stainless steel
Sterilization method: Manual situ sterilization(SIP options)
Control system: Siemens PLC, industrial LCD touch computer control, parameters can be set to store, can be remotely connected to the computer through the USB interface, the data
Working volume 250 to 400 liters and The main material of the fermenter is made by 316Lstainless steel. The design pressure: P(inside)= 0.3Mpa, P(interlayer)= 0.3Mpa, The polishing precision: Ra0.4～0.6, the ratio between diameter and height: 1:2.6
Stirring system
Mechanical sealing system at the top of the fermenter; high-powered stirring oar, antifoam oar; AC electrical machine: infinite speed variation; rpm in the fermenter: 70~500rpm±1%,The height of the stirring oar can be adjusted, too.
Temperature detection and control
Heat by thermostatic water tank and circulate by circulating pump(Grundfos, Denmark), autocontrol (cooling water+5℃)~65℃±0.1℃，temperature probe(US)
pH detection and control
2.00-12.00±0.05pH, autocontrol by adding acid and base, pH sensor (Mettler, Switzerland) and shielding lead (Mettler, Switzerland). fermenter is autocontrolled with peristaltic pump
DO detection and control
0-150±3% displaying precision 0.1%, sensor (Mettler, Switzerland) and shielding lead(Mettler, Switzerland)
Feed control
fermenter uses peristaltic pump (Longer) to add meterials
Antifoam control
Tested by sencor and added antifoamer by peristaltic pump
Intake control (air): manual control rotameter
pressure detection and control
Pressure Control: Pressure meter and digital display and digital remote control, pressure control
we are compliant fermentation equipment for research, development, pilot and production applications. Our Factory in China. We provide the fermentor products including : Stainless steel fermenter, Pilot combo stainless steel fermentor, glass fermentor, fermenter, Multi-Union fermenter，Multi-stagefermentation tank，and shaking Incubators , Positive join stainless steel fermentor.
Technical parameter:
Material and brand of key components
|
No. |
Key component |
Material and brand |
| 1 | Vessel system | The cylinder designed by our company uses stainless steel |
| 2 | Speed Motor | Germany |
| 3 | Speed regulator | Fuji, Japan |
| 4 | Solenoid valve | Taiwan |
| 5 | Aeration filter | 0.2μm, Dominica United Kingdom |
| 6 | Breathing filter | 0.2μm, SARTORIUS, Germany |
| 7 | pH electrode | Hamilton /Mettler-Toledo, Switzerland |
| 8 | DO electrode | Hamilton /Mettler-Toledo, Switzerland |
| 9 | Peristaltic pump | Lange （United States） |
| 10 | PLC | Siemens Germany |
| 11 | LCD touch screen | GCMS |
| 12 | Mechanical seal | John Crane United Kingdom |
1. Double end face and cooling lubricaton system 7. Double mechanical seal – Double protection
2. Inactivated exhaust system 8. Pressure regulator
3. Effect of stirring 9. Balance feeding weighing system
4. Thermal mass flowmeter 10. Tank weighing module-mettler
5. Tank cap automatically open system 11. Siemens PLC, schneider intermediate
6. Tank bottom feeding and moving system 12. All kinds of impeller
| For cell cultivation, bacteria, microbial fermentation and etc. | |
| SEQ. | main features |
| 1 | Full automatic equipment system including automatic cleaning (CIP), automatic sterilzation (SIP) |
| 2 | Flexible and modular-designed system，user-friendly software, ease of operation |
| 3 | With rich experience for customers from different countries with mature standard design and optimized customized design |
| 4 | Economical choice and guarantee a trouble free operation and global |
| 5 | Efficient service support and spare part availability for overseas and domestic market |
| Type | KNIK-5/7/10SJ | KNIK-20/30/50 | KNIK-100/150/200SJ | KNIK-250/300/500SJ |
| Total volume | 5/7/10 | 20/30/50 | 100/150/200 | 250/300/500 |
| Maximum loading Amount of liquid | 4/5/8 | 16/21/40 | 70/120/160 | 200/210/400 |
| Materials | 316L stainless steel | |||
| Stirring Methods | the magnetic structure and top driven by the mechanical coupling | |||
| Basic Configuration (optional) | temperature, RPM, PH, DO, antifoam,feed , aeration (manual), Pressure (manual), sip | |||
| Extensible Configuration (optional) | Liquid level control, feed weighing system, multifeeds & online testing of the content of methanol and ethanol, detection of O2 & CO2 exhausts | |||
| Control System | KNIKbio-B. C. S BIO-Process controller | |||
| Electric Power | 220V 2KW | 220V 3KW | 380V 6KW | 380V 8KW |
| Dimension(L*W*H) | 890*660*1600 | 1300*790*1700 | 1400*820*2200 | 2000*1500*2800 |
| Net weight (kg) | 120 | 150 | 250 | 400 |
| The amount of steam for sterilization | 220V 3-6KW | 380V 9-12KW | 380V 18-24KW | 380V 36-45KW |
