| Equipment | Item |
Requirements and manufacturers |
| 5L 50L 500L
fermenter
|
Fermenter body | The size of fermenter takes in general use type as basis and The main material of the fermenter is made by 316L stainless steel. The design pressure: P(inside)= 0.3Mpa, P(interlayer)= 0.3Mpa, The polishing precision: Ra0.4～0.6, the ratio between diameter and height: 1:2.5
5 liter fermenter Work volumes ranging from 2-3.5 Liters 50 liter fermenter Work volumes ranging from 20-35 Liters 500 liter fermenter Work volumes ranging from 200-500 Liters |
| Sterilization method | Auto sterilization | |
| Stirring system | Mechanical sealing system at the top of the fermenter; high-powered stirring oar, antifoam oar; AC electrical machine(SEW): infinite speed variation;rpm in the 500L germ fermenter: 70~400rpm±1%rpm;, The height of the stirring oar can be adjusted, too. | |
| Temperature detection and control | Heat by thermostatic water tank and circulate by circulating pump(Grundfos, Denmark), autocontrol (cooling water+5℃)~70℃±0.1℃，temperature probe(imported) | |
| pH detection and control | 2.00-12.00±0.05pH, autocontrol by adding acid and base, pH sensor (Hamilton /Mettler, Switzerland) and shielding lead (Hamilton /Mettler, Switzerland). | |
| DO detection and control | 0-150±3% displaying precision 0.1%, sensor (Hamilton /Mettler, Switzerland) and shielding lead(Hamilton /Mettler, Switzerland) | |
| Feed control | fermenter uses peristaltic pump (Longer) to add meterials | |
| Antifoam control | Tested by sencor and added antifoamer by peristaltic pump | |
| Aeration detection and control | manual control ventilatory capacity, the precision of nhaler : 0.1μ, displayed by rotameter. The range of flow adjusting of 10000L germ fermenter ：0~20000L/min | |
| pressure detection and control | Manual control exhaust by diaphragm cell valve,and displayed by pressure gauge. | |
| Pipeline and valve | The pipelines and valves which are in contact with the zymotic fluid are made by 316L stainless steel, the valves are diaphragm cell valves | |
| Control system | KNIKBIO S Control System: on-site controller, the next crew for the Siemens S7-200 series PLC control system, it is a mature, stable, universal type of PLC, to suit a variety of automation applications, especially in manufacturing process control Application. Its modular, easy to implement distributed configuration and easy to master and so on, Siemens PLC control center has become a biological reaction process control implementation is economical and advanced control system, the system uses the touch screen display, on-site direct operations (menu type), all English menu and interface; also with the host computer for data transmission, data sampling and display tank, the system view of data analysis, reading settings.
10-inch LCD touch-sensitive screen as the display screen to display complete content-rich, user-friendly screen is simple, easy to operate. And disturbing each other. The control system can adapt to different range of sensors and actuators the output of the input signal with the signal anti-jamming system. Software: Siemens S7+ FORCE CONTORL Data collection and control module: Siemens S200 PLC controller and data collection and control module 1. Control
2. Measurement function: can feeding, bubble enemy, such as measurement of pH 4. Data Processing
5) data storage format is compatible with EXCEL, EXCEL platform can be to deal with 9. show the controller is really running against, the panel instructed the state process system, process hardware, and motion status indicator for understanding the various devices (such as peristaltic pumps, circulating pumps, heating, mixing, etc.) work status, Fault identification can be used as display |
|
Technical parameter:Material and brand of key components
| No. | Key component | Material and brand |
| 1 | Vessel system | The cylinder designed by our company uses stainless steel |
| 2 | Speed Motor | Germany |
| 3 | Speed regulator | Fuji, Japan |
| 4 | Solenoid valve | Taiwan |
| 5 | Aeration filter | 0.2μm, Dominica United Kingdom |
| 6 | Breathing filter | 0.2μm, SARTORIUS, Germany |
| 7 | pH electrode | Hamilton /Mettler-Toledo, Switzerland |
| 8 | DO electrode | Hamilton /Mettler-Toledo, Switzerland |
| 9 | Peristaltic pump | Lange （United States） |
| 10 | PLC | Siemens Germany |
| 11 | LCD touch screen | GCMS |
| 12 | Mechanical seal | John Crane United Kingdom |
| For cell cultivation, bacteria, microbial fermentation and etc. | |
| SEQ. | main features |
| 1 | Full automatic equipment system including automatic cleaning (CIP), automatic sterilzation (SIP) |
| 2 | Flexible and modular-designed system，user-friendly software, ease of operation |
| 3 | With rich experience for customers from different countries with mature standard design and optimized customized design |
| 4 | Economical choice and guarantee a trouble free operation and global |
| 5 | Efficient service support and spare part availability for overseas and domestic market |
| No | Parameter |
Guideline |
Controller data processing function |
| 1 | pH | Displaying range:0.00~14.00±0.01
Auto-control range:2.00~12.00±0.05 Fermentation process control(According to the fermentation time, auto- control pH fluent, at least10 parts) |
pH value historical and trend curve analysis
Acid, alkali dosage curve analysis Batch report form analysis Acid, alkali total dosage record Keep and deliver data |
| 2 | DO | 0-150±3％，Displaying precision: 0.1％
It can be controlled by rev and feeding. Fermentation process control(According to the fermentation time, auto- control DO fluent, at least10 parts) |
DO value historical and trend curve analysis
Batch report form analysis Keep and deliver data |
| 3 | Antifoam | Automatic PID control and alarm
Fermentation process control(According to the fermentation time, auto- control antifoam fluent, at least10 parts) |
Antifoam value historical and trend curve analysis
Foam error state record antifoam total dosage record Batch report form analysis Keep and deliver data |
| 4 | Rev | Automatic enactment and control
Fermentation process control(According to the fermentation time, auto- control rev fluent, at least10 parts) 500L–50-500RPM |
Rev value historical and trend curve analysis
Batch report form analysis Keep and deliver data |
| 5 | Temperature | Temperature of cooling water: +5℃-70℃±0.1℃
Fermentation process control(According to the fermentation time, auto- control temperature fluent, at least10 parts) |
Temperature value historical and trend curve analysis
Batch report form analysis Keep and deliver data |
| 6 | Feeding | PID automatic enactment and control
Fermentation process control(According to the fermentation time, auto- control feeding fluent, at least10 parts) |
Feeding value historical and trend curve analysis
Feeding total dosage record Batch report form analysis Keep and deliver data |
| 7 | Pressure | Manual control, instrument display | |
| 8 | Air flux | Manual control, instrument display |
KNIKbio-Ver8.0 is a data recording and processing as one of the fermentation of special packages, with the English operation interface, data storage and backup, curve analysis, report generation analysis, anomaly analysis, for the fermentation and production process control and data processing bring a lot of convenience.
Main features:
1. Can record the fermentation time, temperature, pH, dissolved oxygen, rotational speed, air flow, pressure, fill quantity, the amount of foam enemy, acid dosage
2. Can be sent under the controlled variable settings and control parameters, remote operation
3. Can be set sequence control
4. Can type off-line parameters, and records show
5. Trend curve, historical curve shows
can display 1-10 (any setting) curve;
– You can select a different start time and the time period (8,24,48,72,96 and 144 hours);
– You can set the upper and lower curves show, in order to improve display resolution;
– You can choose any of several different batches (1-10) curve also shows that for comparative analysis;
– and Y-axis parallel to the secant by the mouse to drag the reading, followed by curve data is activated, the fermentation time (X axis) and the same time the data curve with the secant movement changes;
6. Report Printing
– can show significant 1-12 (any set) data, and print output;
– You can select a different start time and the time and can choose different time intervals (1,5,15,30,60 minutes);
– You can choose any of several different batches (any set) data, and print output,
– To comparative analysis;
7. curve printing
– You can show the curve was printed by color printer;
EXCEL platform can also be carried out in data processing and printing, is very convenient
