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200L 2000L Double mechanical seal airlift fermentor system for microoganism
200L 2000L Double mechanical seal airlift fermentor system for microoganism - Image 2

200L 2000L Double mechanical seal airlift fermentor system for microoganism

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1. Description for 200L and 2000L Mechanical Agitation Fermenter 

Volume Parameter 200L fermenter with agitation 2000Lfermenter with agitation
TTL Volume 206L 2004L
Work Volume 155L 1503L
Tank Diameter 450mm 1000mm
Tank Height 1200mm 2360mm
The ratio of D:H 1:2.66 1:2.36
Agitator-motor (SEW) Germany SEW AC electrical machine.Speed range:50-400rpm，±%1FS
Sterilization mode Have complete and reliable SIP sterilization system, the sterilization temperature and process is fully automatic monitored by computer sterilization program; Sterilization time is calculated by software automatically and remind after sterilization, thereby to reach safe, convenient and rapid sterilization process. Sterilization temperature: 100-130℃.
Tankbody 1. Tank material : SUS316L Jacket material:SUS304

2. The polishing precision: Ra≤0.4μm,

The roughness of electropolishing internal surface reach toRa≤0.4μm. The fillet is formed by Japanese plate hydraulic forming. All nozzles are formed by Japanese tensile method to form. They confirm to GMP and FDA standard.

All elliptical heads are designed according to Japan Standard.
Tank pressure Designed Pressure≤0.25Mpa, Work pressure≤0.2Mpa
Jacket pressure Designed Pressure≤0.35Mpa, Work pressure≤0.3Mpa
Speed regular (VFD) SIEMENS
Mechanical seal Germany EagleBurgmann double mechanical seal
Stirring oar 2 tiered 6 flat-bladed impeller, adjustable .
Foam breaker 1 tier mechanical foam breaker impeller, adjustable
Filtration system Maximum ventilation capacity is designed at 1.5V/VM, the precision filter applies0.2μm sterilizingmembrane filter ofSartorius brand imported from Germany which can meet repeated high temperature sterilization.
Ventilation pressure Manual control the ventilatory by pressure adjustment; indicate the air inflow capacity by rotameter.
Tail gas discharging Efficiently protected from biological pollution by tail gas filter
Culture transferring and inoculation Adopt regular flame inoculation is much safe and aseptic through peristaltic pump, sanitary silicone tube and exclusive plug pins.
Probe ports 4 ports(1 ph port and3 extra ports with blind plugs)
Competitive Advantage

1. Control System for Biological Process

1) System Summary

BIO-4000 industrial control system is researched and developed by our company. Adopt PLC as the control unit to acquire data on site and transfer to the touch screen and PC by RS-485 signal , to realize the parameter setting, sequence control, process control and a comprehensive analysis of the various data, automatic formation of statements, control interface, touch screen direct display of the implementing agencies working conditions and parameters. It enhances the scalability and compatibility of the system because the standard MODBUS protocol port.

Lower Computer System：Adopt PLC as the control unit commands of touch screen or PC are interpreted to corresponding timing signal to directly control the corresponding equipment. Besides, equipment condition data can be converted into digital signal, and make feedback to the touch screen display or PC for further data processing.

l The software control part adopts PID close loop, which has the features of control High precision control, and the low energy consumption.

l The touch screen as a senior control source, possess the functions of password classification management, parameter setting, alarm management, real-time curve display and on-site report.

Specifications

1) Control System 

NO. CONTROL PARAMETER CONTROL INDICATION
1 PH
  • 1. PLC automatic control
  • 2. Apply intellectual expert PID control, with very good control function of PH degree. It is equipped with Switzerland Hamilton gel type exclusive high temperature resistant sensor.
  • Display range: 0.00-14.00ph, control accuracy: ±0.02, resolution ratio:0.01;
  • Automatic acid and alkali feeding, can be fed by PLC automatic accurate control peristaltic pump or manually to ensure the security in material transmission in fermentation.
  • 33. Touch screen display
    • Display content (PH set value, ph real value, auto curve, alarm parameter, acid & alkali feeding peristaltic pump condition)
3 Temperature
  • 1. PLC automatic control, PT100 temperature sensor.
  • 2. Touch screen display
  • Display content (temperature set value, temperature real value, auto curve, alarm parameter, jacket temperature, all working parts running or stop condition, etc.) Temperature up and lower limit set, control accuracy: ±0.2℃; resolution ratio: 0.1℃; test range: 0-160℃.
  • 3. Automatic heating up by electric heating; cooling water automatic cooling down by solenoid valve control. (Denmark Grundfos circulating pump).
4 Speed
  • 1. PLC automatic control
  • 2. Applies AC SEW motor and Simens Inverter.
  • 3. Rotating speed range:
  • 200L:50-450 rpm, ±%1FS
  • 2000L: 50-450 rpm, ±%1FS
  • 4. Touch screen display
  • Display content ( rotating speed set value, real value, auto curve, alarm parameter)
5 Defoaming
  • 1. PLC automatic control
  • 2. Defoamed by efficient mechanical pressing type and liquid level electrode automatic control. The defoaming system is consisted of feeding hole, conveying pipelines, steam sterilization system, peristaltic pump and PLC to ensure the security in material transmission in fermentation. Add defoamer manually is also feasible. Liquid level electrode automatic control defoaming sensitivity: 100-100000Ω..
  • 3. Touch screen display
  • Display content (defoaming sensor and defoaming peristaltic pump working condition)
6 Material feeding
  • 1. PLC automatic control
  • 2. Digital set time-lapse and quantitive control, material feeding quantity display and record, easy for calculation.

1. Main Units For Fermentation System 

NO. NAME Original Remark
1 Tank China  
2 Agitator-motor (SEW) Germany brand, China made SEW
3 Agitation System (axis,oar,etc) China  
4 Double mechanical seal Germany EagleBurgman
5 Viewing Glass, lamp China  
6 Steam flushed sanitary sampling Valve China  
7 Tank bottom diaphragm vavle China  
8 Cabinet, module, transmitter, cable, switch and so on China  
9 HMI (MCGSE) China HMI
10 PLC Germany brand, China made SEIMENS,S7-200
11 PH probe Swiss Hamilton origin
12 Liquid Level probe China  
13 Defoaming probe China  
14 Feeding pin and pin cap China  
15 Glass Bottle China  
16 Silicon Hose China  
17 Circulating Pump Grandfos, China made
18 Filter Germany SARTORIUS
19 Electric Heater China
20 Peristaltic pump China Foam, acid, alkali, molasses (feeding)
21 Solenoid valve for cooling water inlet China Taiwan
22 Air Rotor Flow meter China
23 Stop valve China
24 Check Valve China Cooling, jacket in
25 Air rebreather, acro 37 (Glass bottle) Germany Sartorius
26 Pressure Indicator China  
29 Converter (VFD) for agitator Germany brand China Made SIEMENS
30 Manual diaphragm Valve China  
31 Temperature PT100 ( Tank body) Japan Chip, China assemble  
32 Temperature PT100 (heater) Japan Chip, China assemble  
33 O -RING (silicone gel) China
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