With the attack of various harmful viruses in recent years, the importance and urgency of biosafety have become increasingly prominent, and the biosafety law will be introduced soon, which is expected to further promote biotechnologies such as antibody drugs, biological vaccines, stem cell drugs, and immune cell therapy. Rapid, compliant, and high-quality development.
Nowadays, single-use process technology can be seen everywhere in the field of biopharmaceuticals. It has the advantages of small fixed investment, time saving, low pollution risk, and flexible operation, which can speed up the development and marketing of drugs. It is being adopted and applied by more and more biopharmaceutical companies and research institutions. Tofflon Medical Equipment has launched the CES cell expansion system, which can solve the automation of some one-time process technologies.
CES细胞扩增系统
In the biopharmaceutical single-use process unit, the CES cell expansion system has a wide range of adaptability and application, such as:
Cellular Drug Production
Antibody drug production
In addition to the CES cell expansion system, it can be used for cell drug production and antibody drug production. It can also have specific applications in the fields of biological vaccine production and plasmid production. At the same time, the CES cell expansion system of Tofflon Medical Equipment also has the following advantages.
Better Mixing Performance: With advanced robotic joint sway control technology, the sway is gentler. Make the gas and nutrients mix more evenly, and at the same time, the gentle shaking reduces the damage caused by the shear force to the cells and affects the cell activity.
Sterility: Fully enclosed disposable consumables technology is adopted to ensure sterility in the production process, no risk of cross-infection, and minimize the risk of cell contamination.
Integrity detection: The system has its own integrity detection function, which can test the integrity of the biological bag before use, avoiding the loss caused during use.
Sampling function: The sampling program is set for the rocking speed, rocking angle, mixing time, and sampling angle, which ensures that the cells are mixed more evenly when sampling, and the accuracy of the user’s sampling is guaranteed.
Process monitoring: Precise control and tracking of key culture parameters (rocking speed, angle, temperature, DO, pH, perfusion rate, etc.).
High-density cell culture: According to the amount of cells to be collected, batch and perfusion culture can be performed to obtain higher density cells.
Mobile APP monitoring function: You can monitor the equipment training situation in real time through the mobile phone, and you can check the real-time alarm information and parameter information.
CES Cell Expansion System Performance Parameters
Case Application Note
The CES cell expansion system is mainly suitable for the culture of CHO, HEK293, immune cells (CAR-T, TCR-T, NK, etc.), adherent cell microcarriers (such as MSC), and the amplification of cells and bacteria such as Escherichia coli nourish.
The picture takes CES10S for T cell culture and expansion as an example:
1. Select the appropriate CES-bag cell culture bag.
Choose culture bags of different specifications according to the requirements of the production process. Specifically, it can be based on the final culture volume, whether there is a pH/DO monitoring requirement, and whether perfusion culture is required.
2. Select the corresponding CES-bag pipeline and accessories according to the process requirements.
Prepare the pipeline or pump tube with the corresponding diameter and connector. At the same time, prepare the pipe connecting machine and pipe sealing machine corresponding to the pipe support.
3. Install CES-bag
Calibration of pH and DO sensors, calibration of peristaltic pumps;
Pipeline connection, exhaust heating jacket installation, etc.;
Carry out a pressure-holding test on the bag through its own software system, 25mbar/5min (to avoid bag defects);
After the weighing unit is tared, the basal medium is transported by a peristaltic pump.
4. Turn on the temperature heating function, and continue to ventilate the air for overnight balance.
5. Connect the seed bag and inoculate by gravity or peristaltic pump.
6. Continuous culture, daily sampling and testing, and regular addition of fresh medium according to the number of cells.
The cell growth curve obtained by CES cell expansion system and ordinary incubator culture T cells is shown in the figure below:
According to the results of the cell growth curve obtained by culturing T cells, the growth status of T cells obtained by CES cell expansion system is roughly consistent with the growth status of T cells obtained in ordinary incubators and has better advantages. The normal and stable expansion and culture of T cells can be realized. In addition, the CES cell expansion system has more advantages in sampling efficiency, convenience of medium addition, real-time monitoring, uniformity of cell culture mixing, cell viability, and sterility assurance.