Autoclavable bioreactor vessel,5L (3 – 3.5 liters working volume) and 7 liter (4-5 liters working volume) and 10 liter (5-7 liters working volume)total capacity

Lid Stainless steel 316L With sterile and pressure proof ports

bioreactor Vessel must be a Jacketed Vessel having with the head plate of SS316L. It should have the Temperature Control via circulation of Hot / Cold Water in the Jacket through an inbuilt Thermo circulator unit (working temperature range 8ºC – 80ºC above coolant temperature) The system must be complete to measure and control pH, DO, stirrer speed (20-300rpm), foam control & temperature.

Probes : Impeller Speed, temperature, pH, redox, pO2, level or Foam, gas mixture

Gas Supplement : Air, O2, N2, CO2

Agitation

Mechanical seal, shaft, 2 height adjustable six – blade stirrer, removable baffle cage

Aeration

Auto cleavable sterile filter, check valve, connection piece, aeration tube with ring sparger

Required air pressure approx, 1.5 bar

Ventilation

Reflux cooler, connection piece, auto cleavable sterile filter

Temperature Circuit

Connected to glass double jackets, all four temperature circuits connected by central supply and drain lines.

Probes

Autoclavable temperature probe Pt100 incl. connecting cable

Autoclavable pH-gel-electrode incl. connecting cable

Autoclavable pO2-probe incl. connecting cable

Autoclavable height adjustable antifoam-probe incl. connecting cable

Sampling System

Autoclavable sampling system

Inoculum

Cup for silicone diaphragm

Measurement of Controls

Integrated in stainless steel trunking

Power supply

Main power supply for all electrical circuits, 1 x 220V, 50 Hz

Temperature Control

Including heating finger and cooling water valve in temperature circuit. Temperature controller for heating and cooling, temperature range 4-60C (depending on the available cooling media)

Speed Setting

DC motor with frequency converter, rpm-control unit

pH Control

pH-control to activate the dosage of acid and base (pumps)

pO2 Control

DDC pO2-control to activate the final elements, Cascade control vial stirrer speed and aeration valve

Antifoam Control

DDC antifoam – Control to activate the dosage to antifoam chemicals

Dosage

Aeration Unit

Consists of pressure valve, a rotameter (50-100L/h) a continuous solenoid valve (proportional valve), activated by pO2 controller, a check valve.

Peristaltic Pump with variable speed

10-inch LCD touch-sensitive screen as the display screen to display complete content-rich, user-friendly screen is simple, easy to operate. And disturbing each other. The control system can adapt to different range of sensors and actuators the output of the input signal with the signal anti-jamming system.

Software: Siemens S7+ FORCE CONTORL

Data collection and control module: Siemens S200 PLC controller and data collection and control module

1. Control
① Manual control: You can set the percentage of open valve or a valve opening ② automatic control mode: You can select continuous or PID PID control the switch ③ sequence control: the control of all parameters can be pre-set at least 10 control section, ④ for automatic segmentation remotely control: PC control ⑤ acceptable correlation control: the dissolved oxygen can select speed, air flow rate, tank pressure, control of feeding, etc.; pH can choose to control with acid and alkali

2. Measurement function: can feeding, bubble enemy, such as measurement of pH
3. XY-axis to chart real progress can be any expansion and contraction of graphics display, to help the operator determine the parameters of the interaction between the effects of the fermentation process to quickly modify and adjust

4. Data Processing
1) to show that all the parameters of the trend curve analysis
2) to store, display, analyze all the historical curve parameters
3) can display all the parameters of the batch report
4) copy the data directly with the USB

5) data storage format is compatible with EXCEL, EXCEL platform can be to deal with
5. Controller self-protection function: to set the password, others can not modify the fermentation parameters; and the controller is not lost due to power settings for each parameter
6. Can the process control parameters, each parameter can have 10 block can be set
7. Time display of each parameter recording interval can be adjusted
8. Two parameters can be carried out with the screen settings and calibration

9. show the controller is really running against, the panel instructed the state process system, process hardware, and motion status indicator for understanding the various devices (such as peristaltic pumps, circulating pumps, heating, mixing, etc.) work status, Fault identification can be used as display
10. Each parameter of the transmission are independent modules, without disturbing each other, for easy maintenance
11.PH, DO and other parameters of control and associated controls can be adjusted

Auto-control range:2.00~12.00±0.05

Fermentation process control

(According to the fermentation

time, auto- control pH fluent, at

least10 parts)

Acid, alkali dosage curve analysis

Batch report form analysis

Acid, alkali total dosage record

Keep and deliver data

0.1%

It can be controlled by rev and

feeding.

Fermentation process control

(According to the fermentation

time, auto- control DO fluent,

at least10 parts)

Batch report form analysis

Keep and deliver data

Antifoam

Automatic PID control and alarm

Fermentation process control

(According to the fermentation

time, auto- control antifoam

fluent, at least10 parts)

Foam error state record

antifoam total dosage record

Batch report form analysis

Keep and deliver data

Rev

+5℃-80°C±0.2℃

Fermentation process control

(According to the fermentation

time, auto- control

temperature fluent, at least10

parts)

Batch report form analysis

Keep and deliver data

Temperature

+5C-80°℃±0.2℃

Fermentation process control

(According to the fermentation

time, auto- control

temperature fluent, at least10

parts)

Batch report form analysis

Keep and deliver data

Feeding

control

Fermentation process control

(According to the fermentation

time, auto- control feeding

fluent, at least10 parts)

Feeding total dosage record

Batch report form analysis

Keep and deliver data

Pressure

Air flux