Advantage

1) There is a control group, and conclusions can be obtained efficiently;

2) To achieve the purpose of process verification;

3) Online detection parameters can be obtained in real time;

4) Complicated process control can be realized with the help of software and control system;

5) With the help of software, various indirect parameters can be calculated, and even the calculation of metabolic flow can be realized to understand the physiological indicators of bacteria in real time;

6) Pass air or protective gas to achieve strict anaerobic, semi-aerobic or high oxygen consumption;

7) The different structures of the fermentation tank can be flexibly changed, such as the form of agitating paddles;

8) It can be used as a bridge between shake flasks and large-size fermentation tanks;

Disadvantage

1) High environmental requirements: Because of the small operating space, it is difficult to achieve strict inoculation operations and aseptic sampling operations;

2) High requirements for personnel, not only the ability of single tank parameter analysis, but also the ability of multi-tank comparative analysis;

3) Many auxiliary facilities: need to provide air, cooling water, sterilizer, etc.;

4) Due to the small size, the control accuracy of the refill system is relatively high;

5) Special software is required, which can realize the overlap of parameters between several parallel tanks at the same time according to customer requirements, and even requires the overlap of historical batch data of different tanks.

Apply to

1) Process verification;

2) Process development;

3) Establish the coupling relationship between the parameters and determine the fermentation process.

4. It can be realized by using 4 parallel fermentation tanks

4.1) Test the influence of different stirring forms on the fermentation process and determine the appropriate KLa and stirring forms;

4.2) Choose 4 better media in the shake flask test, and use parallel fermentation tanks for verification;

4.3) Realize DO control strategy;

4.4) Replenishment control strategy when testing different RQ values;

4.5) Stirring speed test at different times in the early stage to verify energy saving;

4.6) Verify the production capacity of the 4 superior strains;

4.7) Study the changes of metabolic flow under 4 processes;

4.8) Verify the production status of 4 kinds of inoculum;

4.9) Verify the production situation of 4 kinds of inoculation timing